PXBioVisioN’s approach is to identify unique peptides in complex biological mixtures that can be unambiguously correlated to biological events in order to validate novel drug targets and predict drug response. We offer our knowledge of more than 10 years in custom tailored peptide research projects that include:
• patient stratification • drug profiling • sample quality assessment • characterization of animal models
We optimize the set-up of your research project, including study design, sample provision, project management and quality management. Our unparalleled sample preparation service is the prerequisite for the subsequent peptide analysis using methods of chromatography and mass spectrometry. The mass spectrometric data are visualized and processed by Spectromania, the powerful tool for handling large data sets. For peptide identification relevant mass spectrometric signals are analyzed by means of ESI-MS.
PXBioVisioN has many years of experience in pre-clinical and clinical project management to lead your project to success. Our deep knowledge of the applicable quality systems (GxP, ISO 9001) assures an optimal result.
PXBioVisioN's peptide extraction services offer enrichment solutions for the low molecular weight proteome, the peptidome or substances (e.g. drugs) in the same molecular weight range. With a more than 95% of total protein removal, sample volumes between 100 microliter and 1 milliliter, substances in a mass range up to 15 kDa can be explored. In addition to body fluids like blood plasma, serum, urine, CSF or synovial fluid, the unique approach enrich peptides in tissues and cell culture.
All work is being done in our labs in Hannover, Germany. Just send us your samples and you will receive a concentrate that is depleted of proteins ready to run your own downstream analysis.
Improvements made it possible that this technology can be applied not only to peptides but also to enrich drugs from complex body fluids like plasma or serum.
PXBioVisioN has developed a Peptidomics platform to display and interpret peptide patterns in complex body fluids and tissues of various origins. Peptide profiles are systematically and comprehensively measured, compared and interpreted in an appropriate biological context to elucidate knowledge about the biological processes. When applied to relevant disease models, Peptidomics-Technologies have the potential to uncover previously unrecognized factors that may contribute to the pathophysiology.
PXBioVisioN combines state-of-the-art liquid chromatographic separation and mass spectrometry (LC-MS) to separate and profile peptides in biological samples with a high accuracy within the molecular mass range between 950 and 15,000 Da. The LC-MS data are converted into reliable profiling data by proprietary software tools. The integrated Peptidomics data analysis platform Spectromania supports full-resolution visualization of large data sets and full integration of all experimental and process data. The process is supported by rigorous QC/QA measures and is auditable.
Samples are separated by means of liquid chromatography (reversed phase high pressure liquid chromatography) and peptides eluting from the HPLC column are collected into fractions. Each fraction is subjected to MALDI-TOF-MS (matrix-assisted laser desorption/ionization time-of-flight mass spectrometry) and the mass spectra are analyzed.
For the identification of peptides, peaks from individual HPLC fractions can be subjected to nESI-qTOF-MS/MS or MALDI-TOF/TOF-MS sequencing, resulting in peptide fragment spectra. These spectra serve to identify the corresponding peptide sequence by remote database searching.
For MS/MS experiments precursor selection are performed by ‘‘on-the-fly’’ spectra evaluation of the MS spectra. Fragmentation of native peptides can be carried out by collision induced dissoziation. MS/MS spectra are subsequently noise filtered and peak de-isotoped before submission of the fragmentation to a database search engine for peptide identification.
PXBioVisioN helps you to find an appropriate study design for your research project. Since quality controlled sample provision is crucial for the outcome of your study PXBioVisioN provides you a detailed protocol to ensure highest quality of the biological samples.
Since we are accustomed to work in a multi-project environment we developed Proiectus, a multi-user-software that enables an easy operation of several parallel projects. This convenient tool is now ready to ease the management of your project portfolio, too. For more information read here.